| 000 | 02708namaa2200445uu 4500 | ||
|---|---|---|---|
| 001 | doab84064 | ||
| 003 | oapen | ||
| 005 | 20260305123949.0 | ||
| 006 | m o d | ||
| 007 | cr|mn|---annan | ||
| 008 | 220610s2020 xx |||||o ||| 0|eng d | ||
| 020 | _a978-1-0716-0219-5 | ||
| 020 | _a9781071602188 | ||
| 020 | _a9781071602195 | ||
| 020 | _a9781071602218 | ||
| 024 | 7 |
_a10.1007/978-1-0716-0219-5 _2doi |
|
| 040 |
_aoapen _coapen |
||
| 041 | 0 | _aeng | |
| 042 | _adc | ||
| 072 | 7 |
_aPS _2bicssc |
|
| 072 | 7 |
_aPSB _2bicssc |
|
| 720 | 1 |
_aMaiato, Helder _4edt |
|
| 245 | 0 | 0 |
_aCytoskeleton Dynamics _bMethods and Protocols |
| 260 |
_bHumana Press _c2020 |
||
| 300 | _a1 online resource (390 p.) | ||
| 336 |
_atext _btxt _2rdacontent |
||
| 337 |
_acomputer _bc _2rdamedia |
||
| 338 |
_aonline resource _bcr _2rdacarrier |
||
| 490 | 1 | _aMethods in Molecular Biology | |
| 506 | 0 |
_aFree-to-read _fUnrestricted online access _2star |
|
| 520 | _aThis volume details comprehensive state-of-the-art methods on actin microfilaments and microtubules and how they work to achieve different cellular functions in different cellular contexts. Chapters guide readers through protein purification, in vitro reconstitution of several cytoskeleton properties, analyses of microtubule- and actin-based structures, functional dissection of post-translational modifications, and roles in several biological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cytoskeleton Dynamics: Methods and Protocols aims to provide a wide range of experimental approaches and be an invaluable resource for present and future generations of cytoskeleton researchers. The chapter "Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. | ||
| 540 |
_aAll rights reserved _uhttp://oapen.org/content/about-rights |
||
| 546 | _aEnglish | ||
| 650 | 7 |
_aBiochemistry _2bicssc |
|
| 650 | 7 |
_aBiology, life sciences _2bicssc |
|
| 653 | _amicrotubules; golgi-mediated microtubule organization; protein purification; in vitro reconstitution; cytoskeleton properties; nuclear migration | ||
| 720 | 1 |
_aMaiato, Helder _4oth |
|
| 793 | 0 | _aDOAB Library. | |
| 856 | 4 | 0 |
_uhttps://directory.doabooks.org/handle/20.500.12854/84064 _70 _zFree-to-read: DOAB: description of the publication |
| 999 |
_c92915 _d92915 |
||