02404namaa2200469uu 4500001001000000003000600010005001700016006001900033007001500052008004100067020002200108020001800130020001800148020001800166024003500184040001700219041000800236042000700244072001500251720002300266245004300289260002600332300003100358336002600389337002600415338003600441490003300477506005100510520092900561540006301490546001201553650003501565653005801600720002501658720002501683720002301708720002601731720002601757793001801783856011601801999001701917doab64609oapen20260305123949.0m     o  d        cr|mn|---annan210415s2020    xx |||||o     ||| 0|eng d  a978-1-0716-0294-2  a9781071602935  a9781071602942  a97810716029667 a10.1007/978-1-0716-0294-22doi  aoapencoapen0 aeng  adc 7aPS2bicssc1 aMichels, Paul4edt00aTrypanosomatidsbMethods and Protocols  bSpringer Naturec2020  a1 online resource (823 p.)  atextbtxt2rdacontent  acomputerbc2rdamedia  aonline resourcebcr2rdacarrier1 aMethods in Molecular Biology0 aFree-to-readfUnrestricted online access2star  aThis volume explores the latest methods used by researchers to study different trypanosomatid parasites. These methods cover numerous disciplines, from organismal biology to molecular mechanism. The chapters in this book cover topics such as high-throughput sequencing; next-generation analysis of trypanosomatid genome stability and instability; DNA repair in cell extracts; ribosome profiling; and the use of CRISPR/Cas9 technology for gene editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.    Cutting-edge and practical, Trypanosomatids: Methods and Protocols is a valuable resource for any researcher working with trypanosomatids and trypanosomatid-borne diseases.  aAll rights reserveduhttp://oapen.org/content/about-rights  aEnglish 7aBiology, life sciences2bicssc  ainfectious disease; trypanosomatid parasites; biology1 aGinger, Michael4edt1 aGinger, Michael4oth1 aMichels, Paul4oth1 aZilberstein, Dan4edt1 aZilberstein, Dan4oth0 aDOAB Library.40uhttps://directory.doabooks.org/handle/20.500.12854/6460970zFree-to-read: DOAB: description of the publication  c92916d92916