02404namaa2200469uu 4500 doab64609 oapen 20260305123949.0 m o d cr|mn|---annan 210415s2020 xx |||||o ||| 0|eng d 978-1-0716-0294-2 9781071602935 9781071602942 9781071602966 10.1007/978-1-0716-0294-2 doi oapen oapen eng dc PS bicssc Michels, Paul edt Trypanosomatids Methods and Protocols Springer Nature 2020 1 online resource (823 p.) text txt rdacontent computer c rdamedia online resource cr rdacarrier Methods in Molecular Biology Free-to-read Unrestricted online access star This volume explores the latest methods used by researchers to study different trypanosomatid parasites. These methods cover numerous disciplines, from organismal biology to molecular mechanism. The chapters in this book cover topics such as high-throughput sequencing; next-generation analysis of trypanosomatid genome stability and instability; DNA repair in cell extracts; ribosome profiling; and the use of CRISPR/Cas9 technology for gene editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Trypanosomatids: Methods and Protocols is a valuable resource for any researcher working with trypanosomatids and trypanosomatid-borne diseases. All rights reserved http://oapen.org/content/about-rights English Biology, life sciences bicssc infectious disease; trypanosomatid parasites; biology Ginger, Michael edt Ginger, Michael oth Michels, Paul oth Zilberstein, Dan edt Zilberstein, Dan oth DOAB Library. https://directory.doabooks.org/handle/20.500.12854/64609 0 Free-to-read: DOAB: description of the publication 92916 92916